A rapid method of non radioactive Northern blot analysis
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چکیده
منابع مشابه
A rapid method of non radioactive Northern blot analysis.
The electrophoretic determination of RNA size by Northern blot analysis is an important procedure that has required to date the use of radiolabelled cDNA and cRNA probes. Here, we describe a new method which allows the size of an mRNA to be visualized by use of oligonucleotide probes directly labelled with alkaline phosphatase (AP). One advantage compared to radiolabelled probes is that AP-labe...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.14.4291